Part:BBa_K3726046
BOH1_C_Lv1
This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726014” CDS_Lv0_BOH_1_C , the last two enzymes of synthetic butanol biosynthesis operon.
The part in its upstream region, has the connector 5'Con3 “K2560067” that will allow the assembly of a Lv2 construct following the marburg collection standard. In its downstream region has a homology region “BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) for homologous recombination within the genome of PCC 11801.
Likewise, a Kanamycin resistance cassette is included downstream the transcriptional unit region.
Expression is controlled by the medium-strong synthetic constitutive promoter J23119 “BBa_K2560031”and by the synthetic RBS, RBS* “BBa_K3726093”, which has demonstrated its increased ribosome binding strength in Synechocystis PCC6803 and other cyanobacteria in comparison with other heterotroph-derived RBS.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1723
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 431
Illegal NheI site found at 454
Illegal NheI site found at 729
Illegal NheI site found at 957
Illegal PstI site found at 1723 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2057
Illegal BglII site found at 2174
Illegal BglII site found at 4593
Illegal XhoI site found at 3277
Illegal XhoI site found at 4120 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1723
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1723
- 1000COMPATIBLE WITH RFC[1000]
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